Harbour technology

Transgenic mice can be engineered so that functional endogenous murine antibody gene expression is reduced or eliminated, permitting the preferential expression of introduced transgenes encoding human H2L2 antibodies and human HCAb’s or camelised human HCAb’s.

Harbour Antibodies has developed proprietary transgenic mouse strains capable of efficiently producing human HCAbs, camelised human HCAbs and human H2L2 antibodies following antigen challenge, in the absence of endogenous murine H2L2 antibodies.

H2L2 – Human Monoclonal Antibody Mouse Platform

• • Mouse HC & kappa KO
  • Human H & K random insertion
  • Displays key characteristics upon immunization
  • Hypermutation
  • Affinity maturation
  • Class switching etc
  • pM affinities (biacore)

HCAb – Heavy Chain only antibody Mouse Platform

• Mouse HC knockout
• 3 lines generated V4, V18 & V8
• Generating tetravalent Abs
• Key characteristics:
  • Hypermutations
  • Diff J usage
  • Class switching